Comparative Evaluation of Behaviors of Three Naturally Occurring Products, Namely Propolis, Milk, and Egg Albumin When Used as Storage Media in Extracted Teeth for Orthodontic Purpose

Authors

1 Department of Pedodontics and Preventive Dentistry, Government Dental College and Hospital, Aurangabad, Maharashtra

2 Department of Pediatric and Preventive Dentistry, College of Dental Sciences and Hospital, Amargadh, Saurashtra, Gujarat, India

Abstract

Background: The reasonably successful prognosis in retaining avulsed tooth in its respective position depends on the maintenance of the viability of the periodontal ligament (PDL) cells during the replantation procedure. Various synthetic media have been tried for preserving the viability of PDL cells with considerable success. However, easy availability of these media had been a problem at various occasions. Hence, an attempt was made to test the properties of easily available natural products such as Propolis, milk, and egg albumin. Aim: This study was aimed at comparing the behaviors of three naturally available media, namely propolis, milk, and egg albumin when used as the storage media in preserving the viability of traumatized periodontal cells in an avulsed tooth. Materials and Methods: A total of 50 premolars with closed root apices indicated for the extractions for orthodontic treatment were selected. Initially, these teeth in the experimental group were stored dry immediately after the extraction for 30 minutes, and then, immersed in the respective storage media for 45 minutes. The teeth in the positive control group were assessed immediately after the extraction, whereas the negative control teeth were bench dried for 8 hrs. All the five group  samples were then incubated with collagenase and phosphate‑buffered saline for 30 minutes, centrifuged and labeled with 0.5% trypan blue for the determination of cell viability. The cells were counted under the light microscope. The statistical analysis was carried out by applying the unpaired t‑test. Results: No statistically significant difference was observed in viable PDL cell counts when compared amon propolis, milk, and egg albumin. Conclusions: Propolis, milk, or egg albumin could be a good naturally available storage media for avulsed teeth.

Keywords

References

1. Andreasen JO, Andreasen FM. Dental Textbook and Color Atlas of Traumatic Injuries to the Teeth. Copenhagen: Munksgarrd; 1994. p. 383‑419. 
2. World Health Organization. Application of the International Classification of Disease and Stomatology. Geneva: World Health Organization; 1992. 
3. Khinda VIS, Kaur G, Brar GS, Kallar S, Khurana H. Clinical and practical implications of storage media used for tooth avulsion. Int J Clin Pediatr Dent 2017;10:158‑65. 
4. Sangappa SK, Kumar AP, Shrivastava SP. Extra‑alveolar storage media for teeth: A literature review. Int J Adv Res 2014;2:963‑72. 
5. Oswald RJ, Harrington GW, Van Hassel HJ. A postreplantation evaluation of air‑dried and saliva‑stored avulsed teeth. J Endod 1980;6:546‑51. 
6. Chamorro MM, Regan JD, Opperman LA, Kramer PR. Effect of storage media on human periodontal ligament cell apoptosis. Dent Traumatol 2008;24:11‑6. 
7. Andreasen JO. Effect of extra‑alveolar period and storage media upon periodontal and pulpal healing after replantation of mature permanent incisors in monkeys. Int J Oral Surg 1981;10:43‑53. 
8. Ashkenazi M, Sarnat H, Keila S. In vitro viability, mitogenicity and clonogenic capacity of periodontal ligament cells after storage in six different media. Endod Dent Traumatol 1999;15:149‑56. 
9. Patel S, Dumsha TC, Sydiskis RJ. Determining periodontal ligament (PDL) cell vitality from exarticulated teeth stored in saline or milk using fluorescein diacetate. Int Endod J 1994;27:1‑5. 
10. Blomlof L, Lindskog S, Andersson L, Hedstrom KG, Hammarstrom L. Storage of experimentally avulsed teeth in milk prior to replantation. J Dent Res 1983;62:912‑6. 
11. Khademi AA, Atbaee A, Razavi SM, Shabanian M. Periodontal healing of replanted dog teeth stored in milk and egg albumen. Dent Traumatol 2008;24:510‑4. 
12. Gopikrishna V, Baweja PS, Venkateshbabu N, Thomas T, Kandaswamy D. Comparison of coconut water, propolis, HBSS, and milk on PDL cell survival. J Endod 2008;34:587‑9. 
13. Bankova VS, Popov SS, Marekov NL. A study of flavonoids of propolis. J Nat Prod 1983;46:471‑4.
14. Saluja KS, Anegundi R. Assessment of viability of human periodontal ligament cells in different fat content of Milk at different time intervals. Int J Contemp Med Res 2016;3:1376‑9. 
15. Pileggi R, Dumsha TC, Nor JE. Assessment of posttraumatic PDL cells viability by a novel collagenase assay. Dent Traumatol 2002;18:186‑9. 
16. Jain D, Dasar PL, Nagarajappa S. Natural products as storage media for avulsed tooth. Saudi Endod J 2015;5:107‑13. 
17. Ozan F, Polat ZA, Er K, Ozan U, Değer O. Effect of propolis on survival of periodontal ligament cells: New storage media for avulsed teeth. J Endod 2007;33:570‑3. 
18. Warren Strober. Current protocols in Immunology. A.3 B.1-A.3 B.2 Appendix 3B Basic protocol. Common Immunologic Techniques A.3 B.1 Trypan blue exclusion test of cell viability. Supplement 21. John Wiley and Sons, Inc 1997. 
19. Martin MP, Pileggi R. Aquantitative analysis of propolis – A promising new storage media following avulsion. Dent Traumatol 2004;20:85‑9. 
20. Ahangari Z, Alborzi S, Yadegari Z, Dehghani F, Ahangari L, Naseri M. The effect of propolis as a biological storage media on periodontal ligament cell survival in an avulsed tooth: An in vitro study. Cell J 2013;15:244‑9. 
21. Mahal NK, Singh N, Thomas AM, Kakkar N. Effect of three different storage media on survival of periodontal ligament cells using collagenase‑dispase assay. Int Endod J 2013;46:365‑70. 
22. Casaroto AR, Hidalgo MM, Sell AM, Franco SL, Cuman RK, Moreschi E, et al. Study of the effectiveness of propolis extract as a storage medium for avulsed teeth. Dent Traumatol 2010;26:323‑31. 
23. Babaji P, Melkundi M, Devanna R, Suresh BS, Chaurasia VR, Gopinath PV. In vitro comparative evaluation of different storage media (hank’s balanced salt solution, propolis, aloe vera and pomegranate juice) for preservation of avulsed tooth. Eur J Dent 2017;11:71‑5. 
24. American Association of Endodontists. Recommended Guidelines for the Treatment of the Avulsed Permanent Tooth. Chicago, II: American Association of Endodontists; 1995. 
25. Gamsen EK, Dumsha TC, Sydiskis R. The effect of drying time on periodontal ligament cell vitality. J Endod 1992;18:189. 
26. Harkacz OM, Carnes DL Jr., Walker WA. Determination of periodontal ligament cell viability in the oral rehydration fluid gatotade and milk of varying fat content. J Endo 1997;23:687‑90. 
27. Goswami M, Chaitra T, Chaudhary S, Manuja N, Sinha A. Strategies for periodontal ligament cell viability: An overview. J Conserv Dent 2011;14:215‑20. 
28. Rozenfarb N, Kupietzky A, Shey Z. Milk and egg albumen are superior to human saliva in preserving human skin fibroblasts. Pediatr Dent 1997;19:347‑8.

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History

  • Receive Date: 08 November 2020
  • Accept Date: 08 November 2020

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